FIG 2.

Quantification of salicylic acid (SA), cinnamic acid (CA), jasmonic acid (JA), and precursors in PMV- and PMV+SPMV-infected Brachypodium. Levels of SA (A), CA (B), trans- and cis-JA (C), and trans- and cis-12-OPDA (D) were determined using GC-MS. The fresh weight (FW) leaf samples were collected at three different stages of disease progression (early stage I, 7 dpi; mid-stage II, 14 dpi; late stage III, 21 dpi), as described previously (24). The y axes represent average contents of respective metabolites from five biological replicates. The error bars represent standard errors of means (n = 3 and n = 4 for early and for mid and late stages, respectively). *, P ≤ 0.05; **, P ≤ 0.01 between mock- and virus-infected samples as determined by two-sample t test (one-tailed). M, mock; P, PMV; PS, PMV+SPMV; OPDA, 12-oxo-phytodienoate.