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. 2021 Feb 23;12(1):e03695-20. doi: 10.1128/mBio.03695-20

FIG 4.

FIG 4

Exogenous wild-type VP4 increases the efficiency of viral genome translocation. (A and B) Heat maps of synchronized RNA translocation events observed for PV with wild-type VP4-N45 and mutant VP4-T28G as indicated. (C) Comparison of the observed average fold change in intensity of PV-RDL complexes with and without exogenous wild-type and mutant VP4 45-mer at 37°C. Shading indicates the standard deviation of the data points. (D) Distribution of maximum fold change in intensity of PV-RDL complexes observed after 10 min of viral RNA translocation. Error bars indicate standard deviations. (E and H) Distributions of translocation times observed in PV-RDL with wild-type and mutant exogenous VP4 45-mer, respectively. Black lines represent the gamma fit. (F and I) Distributions of individual translocation rates indicating the fold change in intensity per minute. (G and J) Distributions of lag times with corresponding gamma fits (black) indicated.