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. 2021 Sep 2;41(11):2823–2836. doi: 10.1161/ATVBAHA.120.315766

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© 2021 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

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Figure 1. — Change in the nuclear magnetic spectroscopy metabolomic profile during the 3-wk overfeeding of unsaturated fat (UNSAT), saturated fat (SAT), and simple carbs (CARB). Metabolomic analysis was performed from plasma samples collected at baseline and at the end of the intervention. The log-transformed metabolite concentrations were compared using paired 2-sided Student t test with false discovery rate method described by Benjamini and Hochberg. Differences were calculated by subtracting the mean before-value from the mean after-value. Thus, a positive difference indicates an increase and a negative difference a decrease in the mean metabolite concentration or proportion. UNSAT, n=11; SAT, n=13; CARB, n=12. ApoA1 indicates apolipoprotein A1; CARB, simple carbs; HDL, high-density lipoprotein; and SFA%, percentage of saturated fatty acids.