Fig. 1.
Biochemical characterization and antigen-binding activities of recombinant H4-IgG1-4 produced in ΔXTFT. (A) Purified H4-IgG1-4 separated by reducing and nonreducing SDS/PAGE (Coomassie brilliant blue stained), 4 µg protein was loaded at each lane. (B) LC-ESI-MS/MS–derived glycosylation profiles of purified H4-IgG1-4. Bars represent the relative abundance (%) of glycoforms present at the conserved Fc glycosite. (C) ELISA-binding activity EC50 values in nanomoles of purified H4-IgG1-4 to SP using antibodies against κ-LC for detection.