Skip to main content
. Author manuscript; available in PMC: 2021 Oct 25.
Published in final edited form as: Cancer Res. 2019 Nov 19;80(2):263–275. doi: 10.1158/0008-5472.CAN-19-0342

Figure 2: Co-culture involving dispersed cancer cells (MCF7), monocytes (THP-1), and endothelial cells.

Figure 2:

(A) Confocal sections of dispersed cancer cells (green) and monocytes (red) in the interior of the bi-layer GelMA hydrogel at Day 0. XZ section along with XY section at vertical positions (Z1-Z3) show homogeneously distributed cancer cells and monocytes. The dashed white circle indicates the boundary of the inner gel compartment. Vertical scale bar: 20 μm. Horizontal scale bar: 50 μm. (B) Time course brightfield and fluorescent images of cell-laden bi-layer GelMA hydrogel. The interior hydrogel contains a mixture of cancer cells and monocytes (green) while the exterior contains endothelial cells. The boundaries of the interior and exterior hydrogels are lined with red and blue circles, respectively. Scale bar: 200 μm. (C) Quantification of normalized monocyte and cancer cell numbers within the GelMA hydrogel as a function of culture time.