Table 2.
Primer sequences used in the real-time PCR1.
| Gene | Sequences (5′-3′) | Product size, bp | References |
|---|---|---|---|
| CD11b | F: GTCATTGGGGTGGGAGATG R: TCAGCAGGGGGCTTAGATG |
80 | Yan et al., (2011) |
| CD14 | F: CTCAGCGTGCTTGATCTCAG R: AAGGGATTTCCGTCCAGAGT |
98 | Yan et al., (2011) |
| CD68 | F: CAGGGGACAGGGAATGACT R: CCAAGTGGTGGTTCTGTGG |
144 | Yan et al., (2011) |
| MyD88 | F: ATGGTGGTGGTTGTCTCTGAC R: GGAACTCTTTCTTCATTGGCTTGT |
133 | Jiang et al., (2017) |
| TLR-2 | F: CAAGAGGAAGCCCAGGAAG R: TGGACCATGAGGTTCTCCA |
112 | Yan et al., (2010) |
| TLR-4 | F: TGCTGGCTGCAAAAAGTATG R: CCCTGTAGTGAAGGCAGAGC |
139 | Yan et al., (2010) |
| TGFβ | F: AAAAGAACTGCTGTGTTCGTCA R: GACCTTGCTGTACTGTGTGTCC |
149 | Wang et al., (2012) |
| IL-6 | F: AGGAAAAAGATGGATGCTTCCA R: GACCAGCAGTGGTTTTGATCAA |
122 | Herdrich et al., (2010) |
| IL-1β | F: CGTCTTCCTGGGACGTTTTAG R: CTGCGTATGGCTTCTTTAGGG |
97 | Yang et al., (2016) |
| NF-κB | F: ATACGTCGGCCGTGTCTAT R: GGAACTGTGATCCGTGTAG |
125 | Zhang et al., (2019) |
| TNF-α | F: ACACCATGAGCACCAAAAGC R: AGGCACAAGCAACTTCTGGA |
109 | Puttabyatappa et al., (2019) |
| β-actin | F: CACCGCAAATGCTTCTAGGC R: ACGAGGCCAATCTCATCTCG |
96 | Dong et al., (2020) |
CD = cluster of differentiation; MyD88 = myeloid differentiation factor 88; TGFβ = Transforming growth factor β; TLR = toll-like receptor; IL = interleukin; TNF-α = tumor necrosis factor α; NF-κB = nuclear factor kappa B (p65); F = forward; R = reverse.