Figure 4.

Automated in meso crystal harvesting and cryo-cooling

(A) Schematic representation of the CrystalDirect harvesting process: the laser cuts an aperture in the drop and the robot gently removes the excess precipitant solution by aspiration, then the laser-based film excision is applied and the film piece containing the sample is automatically glued to a data collection pin and cryo-cooled. Samples are then transferred to a high-capacity cryogenic storage system (not shown).

(B–E) Examples of ADIPOR2 and ACER3 microcrystals (pink arrows) grown and harvested from CrystalDirect plates. Different scenarios involving harvesting of full boluses or sections of them are presented. (B) Full LCP drop; (C) high-density area; (D) single crystals; (E) single crystals.