Table 2.
Primers used in this study.
| Primer name | Primer sequences (5′-3′) | Target location | RNA-seq result |
|---|---|---|---|
| Primers for biomarker candidate sperm small RNA | |||
| dxRN_1 | CCAAGTCCCAGTTGTCCTGG | chr6 | Down-regulated |
| dxRN_2 | AGGTAGTGAGGGGGAGGAG | chr7 | Down-regulated |
| dxRN_3 | CCTAGATCTCGGGGGTCGTC | chr7 | Down-regulated |
| dxRN_4 | GATCCAAGGGATCAGGGTTGG | chr7 | Down-regulated |
| dxRN_5 | TACTTCCGAGTCTGGCCTGA | chr7 | Down-regulated |
| dxRN_6 | GACGCCGCTAAGATGCTTTC | chr7 | Down-regulated |
| dxRN_7 | GAAGTGGTGGTCACTGTCCC | chr9 | Up-regulated |
| Primers for endgenous control gene | |||
| dxRef_1 | GCTGGAGTGCAGTGGCTATT | chr12 | – |
| dxRef_2 | CTATGTTGCCCAGGCTGGAG | chr12 | – |
| U6_Fw | Commercial primer | – | – |
| U6_Rv | Commercial primer | – | – |
All primers listed in the table except U6 primers were used as forward primers. The reverse primer was a commercially supplied universal primer in Mir-X miRNA qRT-PCR TB Green kits (Takara Bio). The primers for U6 were also included in the kit.