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. 2021 Oct 7;9:671564. doi: 10.3389/fcell.2021.671564

FIGURE 1.

FIGURE 1

P16 deletion improved damage of barrier structure and dysfunction in intestinal epithelium in Bmi-1–/– mice. The experiments were carried out on the 7-week-old Bmi-1–/–, Bmi-1–/–p16–/–, and WT mice. (A) Representative appearances. (B) Body weight (g). (C) Whole view of the intestine. (D) Intestinal length (cm). (E,F) H&E and AB-PAS staining of jejunum, ileum, and colon. (G) Villous length (μm). (H) Villous length relative to crypt depth. (I) The number of Paneth cells relative to the number of crypts. (J) The number of acidic mucoprotein positive cells. (K) Glycoprotein-positive cells relative to the number of villus. (L) Representative micrographs of paraffin-embedded intestinal sections immunohistochemical staining for p53, with hematoxylin staining the nucleus. (M,N) Percentage of p53-positive cells relative to the total cells. Six mice per group were used for experiments. Statistical analysis was performed with one-way ANOVA test. Values are mean ± SEM from six determinations per group, *p < 0.05, **p < 0.01, ***p < 0.001 compared with the WT group; #p < 0.05, ##p < 0.01 compared with the Bmi-1–/– group.