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. 2021 Oct 12;9:753223. doi: 10.3389/fcell.2021.753223

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Copyright © 2021 Vinci, Pedicino, Bonanni, D’Aiello, Severino, Pisano, Ponzo, Canonico, Ciampi, Russo, Di Sario, Montone, Trani, Conte, Grimaldi, Cribari, Massetti, Crea and Liuzzo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Flowchart showing the allocation of the enrolled patient population within each experimental procedure. (A) Among the 104 enrolled patients, 30 were CCS and 74 were NSTE-ACS; (B) The analysis of distribution of circulating monocyte subsets was performed according to CD14 and CD16 expression on 30 CCS and 69 NSTE-ACS patients; (C) 5 new NSTE-ACS patients were enrolled to characterize a novel monocyte subpopulation; (D) 21 of the 69 NSTE-ACS patients underwent OCT investigation and OCT-MØI assessment, resulting in 8 IFC and 13 RFC, respectively; (E) Lastly, 5 CCS and 10 NSTE-ACS patients of the 30 CCS and 69 NSTE-ACS, respectively, were employed for the in vitro LPS-treatment and cytokine multiplex assay. CCS, chronic coronary syndrome, IFC, intact fibrous cap; LPS, lipopolysaccharides; MØI, machrophage infiltration; NSTE-ACS, non ST-elevation acute coronary syndrome; PBMCs, peripheral blood mononuclear cells; RFC, ruptured fibrous cap; w/o, without; w/with. Art images from http://smart.servier.com.