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Identification of a novel subset of circulating monocytes.Gating strategy and monocyte subset identification by flow cytometry in a representative patient. Monocytes were gated in a forward/sideward scatter (FS/SS) dot plot (left plot) and the three monocyte subsets (classical, intermediate, and non-classical) (middle plot) as well as the novel pre-classical monocyte population were identified according to CD14 and CD16 cell surface expression (right histogram). DN = double negative.
