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Primers used for qRT-PCR.
| Forward primer (5′-3′) | Reverse primer (5′-3′) | |
|---|---|---|
| MUC2 | GCTGACGAGTGGTTGGT GAATG |
GATGAGGTGGCAGACAGGAGAC |
| TFF3 | CCGTGGTTGCTGTTTTGAC | GCCTGGACAGCTTCAAAATG |
| ZO-1 | ACCCGAAACTGATGCTGTG GATAG |
AAATGGCCGGGCAGAACTTGTGTA |
| claudin-1 | AGCTGCCTGTTCCATGTACT | CTCCCATTTGTCTGCTGCTC |
| occludin | ACGGACCCTGACCACTATGA | TCAGCAGCAGCCATGTACTC |
| TNF-α | CCCTCACACTCAGATCAT CTTCT |
CTACGACGTGGGCTACAG |
| IL-6 | CTCTGGCGGAGCTATTGAGA | AAGTCTCCTGCGTGGAGAAA |
| GAPDH | GAAGGTGAAGGTCGGAG TCAAC |
CATCGCCCCACTTGATTTTGGA |
