Table 2 –
Gene targets used for qPCR.
| Gene | Accession # | Function | Sequence | Product Length (bp) | Annealing Temp (°C) |
|---|---|---|---|---|---|
| B2m | NM_009735.3 | Housekeeping gene | F: CTGCTACGTAACACAGTTCCACCC R: CATGATGCTTGATCACATGTCTCG |
241 | 55 |
| Rn18s | NR_003278.3 | Housekeeping gene | F: GTAACCCGTTGAACCCCATT R: CCATCCAATCGGTAGTAGCG |
151 | 55 |
| Acp5 | XM_006509946.3 | Codes for TRAP, an enzyme expressed by osteoclasts that breaks down bone mineral | F: CCATTGTTAGCCACATACGG R: ACTCAGCACATAGCCCACAC |
99 | 55 |
| Ctsk | NM_007802.4 | Codes for cathepsin k, a protease expressed by osteoclasts | F: GTTGTATGTATAACGCCACGGC R: CTTTCTCGTTCCCCACAGGA |
75 | 60 |
|
Dmp1 |
XM_006520519.5 | Codes for dentin matrix acidic phosphoprotein 1, an extracellular matrix protein expressed by maturing osteoblasts that orchestrates mineralized matrix formation |
F: CAACTGGCTTTTCTGCGGCAA R: TGGGTGCGCTGATGTTTGCT |
150 | 55 |
| Plin1 | NM_175640.2 | Codes for perilin 1, a lipid droplet protein expressed by adipocytes to prevent lipase activity and highly upregulated by PPARγ |
F: GGGACCTGTGAGTGCTTCC R: GTATTGAAGAGCCGGGATCTTTT |
228 | 55 |