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. 2021 Oct 25;6:126. doi: 10.1038/s41541-021-00387-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Cartoon representation of the interactions between Ab1245 V_H-V_L domains (pink with CDRH3 in cyan) and the tryptophan clasp of gp41 (gp41 in dark gray with Trp residues 623_gp41, 628_gp41, and 631_gp41 in dark blue) with gp120 in light gray. Interacting residues between M100c_HC and the tryptophan clasp shown as sticks. b Cartoon representation of the same view of an unbound protomer of gp41 with the portion of gp41 containing the fusion peptide (red) and fusion peptide proximal region (FPPR, green) interacting with the gp41 tryptophan clasp (same coloring as in (a)). Interactions between M530_gp41 and the tryptophan clasp are shown as sticks. c Surface representation of gp41 (dark gray) and gp120 (light gray) with tryptophan clasp residues in dark blue. Ab1245 CDRH3 is cyan with a stick representation for the Met100c_HC sidechain. d In vitro neutralization assays using IgGs Ab1245 or N6⁶⁶ (positive control for neutralization) against the indicated viral strains. In addition to those listed, Ab1245 was tested in-house against pseudoviruses from strains CE0217, CNE55, JRCSF, Du422, T250-4, Tro, X1632, 246F3, CH119, CE1176, BJOX002000_03_02, 25710, X2278, CNE8, and 398F1 at a top concentration of 500 µg/mL or 1000 µg/mL along with IgG N6 (positive control at a top concentration of 10 µg/mL). N6 was not evaluated in against the strains indicated by a dash because its neutralization potencies were previously published⁶⁶. Whereas N6 exhibited expected neutralization potencies against evaluated strains⁶⁶, Ab1245 exhibited no neutralization activity.