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. 2021 Oct 12;12:724144. doi: 10.3389/fpls.2021.724144

Figure 1.

Figure 1

SPY interacts with CPN20. (A) SPY interacts with CPN20 through TPR domains in yeast two-hybrid assay. Pictures were taken after yeast cells were grown on SD-3/X/A for 4 days. BD, DNA-binding domain; AD, activation domain; SD-3/X/A, SD medium lacking Leu, Trp, and His and adding X-α-Gal and Aureobasidin A; (B), SPY interacts with CPN20 in BiFC assay. Protoplasts were transformed with pairs of indicated vectors and observed under a confocal microscope using identical parameters. AUTO, chloroplast autofluorescence; YFP, the fluorescence of the YFP protein; Merged, merged image of YFP, AUTO, and the bright-field image. (C,D) ABA promotes interaction between SPY and CPN20 in Split-Luc assay. Leaves of tobacco were transformed by infiltration of GV3101 with indicated construct pairs. LUC images were captured by cooled CCD imaging apparatus (C). Relative LUC activities were measured by GLOMAX 96 microplate luminometer. Data are shown as means ± SE measured from 8 leaf disks punched from leaf area injected with GV3101 (D). The concentration was 10 μM when ABA was applied.