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. 2021 Oct 12;12:724144. doi: 10.3389/fpls.2021.724144

Figure 2.

Figure 2

CPN20 is O-fucosylated in mammalian cells and in planta. (A) O-fucosylation sites in CPN20 determined by ETD-MS/MS. A sequence in gray is an O-fucose modified peptide within CPN20 isolated from germinating seeds of CPN20-GFP/35S:HA-SPY. Red letters indicate confirmed O-fucosylating sites. (B) Percentage of IDITO−fucoseVPTO−fucoseGAQIIYSK vs. IDITVPTGAQIIYSK identified by ETD-MS/MS from CPN20-GFP purified from germinating seeds of indicated materials under indicated conditions. The percentage was calculated by spectra counting. The concentration was 10 μM when ABA was applied. (C) ETD MS2 spectrum of the tryptic peptide IDITO−fucoseVPTO−fucoseGAQIIYSK from CPN20-GFP immune-purified from 293T cells ectopically expressing this fusion protein. (D) ETD MS2 spectrum of the tryptic peptide IDITO−fucoseVPTO−fucoseGAQIIYSK from CPN20-GFP prepared from germinating seeds of CPN20-GFP/35S: HA-SPY.