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. 2021 Oct 26;10(10):71. doi: 10.1038/s41389-021-00359-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — A Potential developmental trajectory of CD8+ T cells inferred by analysis with Monocle2. B Dynamic changes in gene expression of CD8+ T cells during the transition (divided into 3 phases), subtypes are labeled by colors (upper panel). C Histogram showing the cell distribution of CD8+ T cells, in SA-ESCC and NACT-ESCC samples. CD8 subtypes labeled by colors. D Histogram showing the cell distribution of SA-ESCC and NACT-ESCC samples. E Heatmap showing the activity of TFs in each CD8+ T cell subtypes in each condition. The TF activity is scored using AUCell. Left, SA-ESCC. Right, NACT-ESCC. F Heatmap showing the activity of immune checkpoints in each T cell subtypes in each condition. Left, SA-ESCC. Right, NACT-ESCC.