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. 2021 Oct 25;4:1223. doi: 10.1038/s42003-021-02757-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b MAFE assay to measure food ingestion after a single compression. The feeding time (a) and food consumption (b) were quantified in each fly (n = 10 per group). Results are shown as mean ± SD. c The defecation assay after a single compression. Thirty flies per group were fed with 5% sucrose containing a blue dye, then placed into 10 separated vials. The blue deposit was counted on each vial after 12 h. The average deposit per fly was calculated for each vial. Results are shown as mean ± SD. d The survival curve after multiple compressions. A total of 97% flies were alive after being released after loading every 2 days from day 0 to day 14 (n = 60 per group), p = 0.03 (HR = 1.65; 95% CI = 1.11–2.45). The Drosophila strain used for all the experiments was the Oregon R (wild type). ns, not significant. See the Methods section for all the experimental details.