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(A) No evidence of cellular senescence was detected by RT-PCR in TGFβ1-stimulated tissue compared to matched controls using a panel of 10 diverse senescence markers (n = 5 donors). (B) A selection of fibrotic markers, COL1A2, COL3A, FN1 and ACT2A quantified by RT-PCR, showed early fibrotic changes in human TGFβ1-stimulated lung tissue as early as day 4, n = 3.
