FIGURE 2.

Differentially expressed genes in human lung parenchyma after 7 days stimulation with TGFβ1 compared to control tissue. (A) A volcano plot identifying the statistically significant genes regulated by TGFβ1 shown as p value versus log₂FC. Out of 84 genes, 36 were significantly upregulated and three downregulated following a 5% false discovery analysis with John D. Storey adjusted p value <0.05 and absolute value of log₂FC ≥ 0.5, depicted by the dotted lines and grey shaded area (results are mean of 32 individual donors). (B) The log₂FC of all 84 genes in individual donors is depicted in this heatmap, with mean log₂FC, and statistical significance indicated. Significance was calculated using a student’s t-test on the ΔΔCT for each gene.