FIGURE 7.

Schematic summary of the most important biophysical determinants for the Osh6 transport. (A) PS is transported along the gradient spontaneously. (B) If PI4P is present in the PS-accepting membrane, Osh6 binding pocket is occupied by PI4P and PS transport is inhibited. (C) Sac1 fails to dephosphorylate PI4P if no negatively charged lipid is present in the PI4P-acceptor membrane. (D) Both the PS and PI4P transports are occurring if the PI4P release to the acceptor membrane is synchronized with the Sac1 action, which is subjected to the PS (or PI, PG) presence in the acceptor membrane.