Table 2.

Primers used for lentivirus-mediated CYP26A1 overexpression in Raw264.7.

Primer name	Sequences (5’ - 3’)
Primers used for amplify Cyp26a1 (1520bp)	F: ATAAGAATGCGGCCGCCGTGCCATGGGGCTC-CCGGCGCTGCT R: CGGGATCCTCAGATATCTCCCTGGAAGTGG
Primers used for plasmid identification (1658bp)	F: CTCAAGCCTCAGACAGTGGT R: ACACCGGCCTTATTCCAAGC
Primers used for PCR identification (WT: 526bp; KO: 99bp; KO-OE: 99bp,334bp)	F: TCTGGGACCTGTACTGTGTGA R: TTCTTTCGCTGCTTGTGCG
Primers used for qPCR identification (218bp)	F: GCTCAAGCTCTGGGACCTGT R: CATTATCCGCGCCCATCACC

F, forward; R, reverse; WT, Raw264.7 wild-type cells; KO, Raw264.7 CYP26A1^−/− cells; KO-OE, CSII-CYP26A1-CYP26A1^−/− Raw264.7 overexpression cells.