Figure 2.
Affinity of rhesus macaque FcγRs for a panel of human IgG1 glycovariants. Equilibrium dissociation constants (KD) of rhesus macaque alleles having unique extracellular domains observed in two independent experiments (rows). Within each experiment, glycovariants were printed in replicate and each replicate is plotted. Results are presented such that each panel emphasizes a different category of glycomodification, including variable fucosylation (F) (A), bisecting N-Acetylglucosamine (B) (B), sialylation (S) (C), and galactosylation (G) (D). A natively glycosylated preparation (black) is plotted along with variants with (+) or lacking (-) the glycan emphasized in that panel. Statistically significant differences were tested using an unpaired t test comparing glycovariants with (+) and without (-) the modification (*p < 0.05, ***p < 0.0005, ****p < 0.0001).