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. 2021 Oct 8;108(10):2006–2016. doi: 10.1016/j.ajhg.2021.08.003

Figure 4.

Figure 4

Spata5l1 is expressed in the inner ear and the brain of rodents/humans

(A) SPATA5L1 is expressed in all cellular subtypes of the human brain (Brain RNA-Seq database).

(B) Representative immunofluorescent labeling of endogenous SPATA5L1 (green) in rat hippocampal neurons in culture highlight the co-localization of the protein within the nuclei of neurons (MAP2), as well as the nuclei of glial cells (red): microglia (IBA1), astrocytes (GFAP), and oligodendrocytes (Olig2). Signal intensity of SPATA5L1 immunolabeling suggests protein expression is higher in neurons compared to glial cells. Arrows indicate the localization of SPATA5L1 in the nuclei of glial cells. All images are projections of confocal optical section stacks. Scale bar: 25 μm.

(C) Spata5l1 is expressed at low levels in hair cells (inner and outer) as well as supporting cells (pillar and Deiter cells) in adult mice (adapted from gEAR portal). HC, hair cells.

(D) Representative immunofluorescent labeling of endogenous SPATA5L1 (green) in Sprague Dawley rat organ of Corti at E20. Immunolabeling shows SPATA5L1 is present in the hair and pillar cells of the organ of Corti. DAPI (blue) and Rhodamine Phalloidin (red) were used for counterstaining the nuclei and the cytoskeleton, respectively. All images are projections of confocal optical section stacks. Scale bar: 10 μm.