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. 2021 Oct 12;11:731595. doi: 10.3389/fcimb.2021.731595

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Copyright © 2021 Fotouhi-Ardakani, Ghafari, Ready and Parvizi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic illustration of primer and probe (nucleotide site variation) in AAP3 gene for diagnostics of Leishmania species using qPCR, arrows of start and end sites showing primer sites and internal arrows showing probe site; difference of nucleotides is specified by dot. The primer and probe sites are shown by red and green arrows, respectively. Sequences are specified by dot except different nucleotides. (A) Specific primers and probes of all species; (B) specific primers and probes of L. major; (C) specific primers and probes of L. tropica.