TABLE 6.
Differentially expressed miRNAs and their targeted mRNAs.
| miRNA | Log2FC | Adjusted p-value (FDR) | Targeted mRNAs |
| Atherosclerosis vs. Control | |||
| miR-193a-5p | 1.66 | <0.001 | ERBB2, IGF2BP1, ING5, MTOR, NLN, PIK3R3, SRR, TFAP2A, TP73, WT1 |
| miR-27a-5p | 1.05 | 0.027 | EGFR, GREM1, MXI1, PEBP1, PPARA, SFRP1 SNAP25, TXN2 |
| miR-320b | 1.19 | 0.002 | DLX5, MYC, NOD2 |
| miR-320d | 1.51 | <0.001 | GNAI1, RBFOX2 |
| miR-483-5p | 1.35 | 0.045 | CKB, ALCAM, FAM160B2, MAPK3, RHOA, SRF, NOTCH3 |
| miR-495-3p | –1 | 0.009 | AKT1, ATP7A, BMI1, RUNX3, FOXC1, HSPA5, MAT1A, MEIS1, PBX3, ABCB1, CCL2, SOX9, HMGA2, SMR3B, PTP4A3, TBC1D9, MTA3 |
| miR-543 | –1.04 | 0.003 | KRAS, MMP7, NOS3, PTK2, TWIST1, HMGA2, MTA1, SIRT1, FBXO11 |
| miR-576-3p | 1.24 | <0.001 | CCND1 |
| Abdominal aneurysm vs. Control | |||
| miR-122-5p | 1.10 | 0.050 | ADAM10, ALDOA, ANK2, ANXA11, RHOA, ATP1A2, AXL, BAX, BCL2L1, BCL2L2, CCNG1, CDK4, CREB1, CYP7A1, DUSP2, EGFR, FUT8, GYS1, HMOX1, IGF1R, IL1A, MECP2, MEF2D, NCAM1, P4HA1, PDK4, PKM, PRKAB1, MAPK11, PTPN1, RAC1, SLC7A1, SRF, ADAM17, TPD52L2, VEGFC, WNT1, PRKRA, SOCS1, TBX19, NUMBL, ENTPD4, AKT3, TRIB1, SPRY2, AP3M2, FOXJ3, PEG10, LPIN1, XPO6, CTDNEP1, SLC7A11, DSTYK, CLIC4, FOXP1, NT5C3A, RAB6B, GALNT10, UBAP2, ZNF395, NOD2, AACS, FUNDC2, RAB11FIP1, NFATC2IP, G6PC3, EGLN3, ACVR1C, FAM117B |
| miR-193a-5p | 1.97 | <0.001 | ERBB2, IGF2BP1, ING5, MTOR, NLN, PIK3R3, SRR, TFAP2A, TP73, WT1 |
| miR-27a-5p | 1.57 | 0.002 | EGFR, GREM1, MXI1, PEBP1, PPARA, SFRP1 SNAP25, TXN2 |
| miR-320b | 1.51 | 0.002 | DLX5, MYC, NOD2 |
| miR-320c | 2.00 | <0.001 | PRDM1, EZH2, GNAI1, IRF4, SMARCC1, XBP1, NOD2 |
| miR-320d | 1.94 | <0.001 | GNAI1, RBFOX2 |
| miR-483-5p | 1.76 | 0.027 | CKB, ALCAM, FAM160B2, MAPK3, RHOA, SRF, NOTCH3 |
| miR-543 | –1.09 | 0.026 | KRAS, MMP7, NOS3, PTK2, TWIST1, HMGA2, MTA1, SIRT1, FBXO11 |
| miR-576-3p | 1.57 | <0.001 | CCND1 |
| miR-629-5p | 1.2 | <0.001 | HNF4A, TRIM33 |
| Coronary heart disease vs. Control | |||
| miR-193a-5p | 1.23 | 0.003 | ERBB2, IGF2BP1, ING5, MTOR, NLN, PIK3R3, SRR, TFAP2A, TP73, WT1 |
| miR-320d | 1.23 | 0.019 | GNAI1, RBFOX2 |
| miR-370-3p | –1.22 | 0.007 | MAP3K8, CPT1A, CTNNB1, GADD45A, FOXM1, FOXO1, FOXO1, MGMT |
| miR-409-3p | –1.24 | 0.006 | AKT1, ANG, CTNND1, ELF2, FGA, FGB, FGG, GAB1, IFNG, MET, MGMT |
| miR-493-3p | –1.22 | 0.017 | RHOC, MXI1, MAP2K7, FZD4, DKK1 |
| miR-495-3p | –1.16 | 0.018 | AKT1, ATP7A, BMI1, RUNX3, FOXC1, HSPA5, MAT1A, MEIS1, PBX3, ABCB1, CCL2, SOX9, HMGA2, SMR3B, PTP4A3, TBC1D9, MTA3 |
| miR-543 | –1.09 | 0.017 | KRAS, MMP7, NOS3, PTK2, TWIST1, HMGA2, MTA1, SIRT1, FBXO11 |
| Carotid stenosis vs. Control | |||
| miR-193a-5p | 1.36 | 0.005 | ERBB2, IGF2BP1, ING5, MTOR, NLN, PIK3R3, SRR, TFAP2A, TP73, WT1 |
| miR-27a-5p | 1.15 | 0.087 | EGFR, GREM1, MXI1, PEBP1, PPARA, SFRP1 SNAP25, TXN2 |
| miR-320d | 1.14 | 0.067 | GNAI1, RBFOX2 |
| miR-335-3p | –1.03 | 0.036 | ESR1, NOS3, PAX6 |
| miR-381-3p | –1.15 | 0.036 | CD1C, GJA1, ID1, NFKBIA, P2RX5, TWIST1, WEE1, HDAC4, TBC1D9, ANO1 |
| miR-493-3p | –1.05 | 0.084 | RHOC, MXI1, MAP2K7, FZD4, DKK1 |
| miR-495-3p | –1.16 | 0.045 | AKT1, ATP7A, BMI1, RUNX3, FOXC1, HSPA5, MAT1A, MEIS1, PBX3, ABCB1, CCL2, SOX9, HMGA2, SMR3B, PTP4A3, TBC1D9, MTA3 |
| miR-543 | –1.08 | 0.045 | KRAS, MMP7, NOS3, PTK2, TWIST1, HMGA2, MTA1, SIRT1, FBXO11 |
| miR-654-3p | –1.14 | 0.039 | CDKN1A |
| Peripheral artery disease vs. Control | |||
| miR-193a-5p | 1.84 | <0.001 | ERBB2, IGF2BP1, ING5, MTOR, NLN, PIK3R3, SRR, TFAP2A, TP73, WT1 |
| miR-199a-5p | –1.14 | 0.002 | ACVR1B, JAG1, APOE, DDR1, CAV1, CD44, CDH1, CDH2, CDKN1C, CTSC, CLTC, CCR7, EDN1, ERBB2, ERBB3, ERN1, ETS1, EZH2, GSK3B, HIF1A, HK2, HSPA5, IKBKB, ITGA3, JUNB, KRAS, LDLR, LIF, SMAD3, SMAD4, MAP3K11, NFKB1, PDE4D, PIK3CD, PSMD9, PTGS2, NECTIN1, SMARCA2, SNAI1, SULT1E1, TGFB2, TGFBR1, UNG,, WNT2, LIN7A, VEGFA, WNT2, FZD4, FZD6, MAP4K3, BECN1, LIN7A, KL, MAFB, PIAS3, ATF6, RAB21, SIRT1, SIRT1, GPR78, RND1, SETD2, DRAM1, DNAJA4, WNK1, ARHGAP12, TMEM54, OSCP1, SLC27A1 |
| miR-215-5p | –1.01 | 0.005 | ACVR2B, ALCAM, XIAP, RUNX1, NID1, RB1, ZEB2, PTPRT, SIGLEC8, CTNNBIP1, WNK1, KDM1B |
| miR-320b | 1.31 | 0.004 | DLX5, MYC, NOD2 |
| miR-320c | 1.22 | 0.045 | PRDM1, EZH2, GNAI1, IRF4, SMARCC1, XBP1, NOD2 |
| miR-320d | 1.42 | 0.007 | GNAI1, RBFOX2 |
| miR-576-3p | 1.46 | <0.001 | CCND1 |
| miR-582-3p | 1.08 | 0.011 | SFRP1, AXIN2, DKK3 |
| miR-629-5p | 1.2 | <0.001 | HNF4A, TRIM33 |
| miR-769-5p | 1.01 | 0.052 | GSK3B, TRAPPC2B |
Targets were determined using the miRTarBase database with annotations supported by strong experimental evidence. Log2FC and FDR were calculated by DGE analysis with DESeq2. Bold-marked miRNAs are exclusively differentially expressed. Log2FC = log2 fold change; B-H p-value = adjusted p-value (Benjamini & Hochberg); FDR = false discovery rate.