. 2021 Oct 12;12:604313. doi: 10.3389/fmicb.2021.604313

Table 1.

Effects of long-term TCS exposure on the relative abundance of the top 8 dominant microbiota in the gastrointestinal tract of zebrafish at phylum level.

		Proteobacteria		Fusobacteria		CKC4		Firmicutes
TCS exposure levels (ng/mL)	n	mean±SD	β(95%CI)	mean±SD	β(95%CI)	mean±SD	β(95%CI)	mean±SD	β(95%CI)
0(DMSO)	8	0.7254 ± 0.1819	Reference	0.1934 ± 0.1920	Reference	0.0013 ± 0.0033	Reference	0.0298 ± 0.025	Reference
blank control	7	0.3535 ± 0.1468	−0.3719(−0.5254, −0.2185)^***	0.3957 ± 0.1810	0.2023(0.0397, 0.3649)^*	0.1819 ± 0.0978	0.1805(0.0771, 0.2840)^**	0.0303 ± 0.0575	0.0004(−0.0948, 0.0957)
0.03	6	0.4293 ± 0.1122	−0.2961(−0.4562, −0.1360)^**	0.3873 ± 0.1014	0.1939(0.0242, 0.3636)^*	0.1616 ± 0.0948	0.1602(0.0523, 0.2682)^**	0.0044 ± 0.0037	−0.0254(−0.1248, 0.0740)
0.3	7	0.5316 ± 0.1417	−0.1938(−0.3472, −0.0404)^*	0.1921 ± 0.1019	−0.0014(−0.1640, 0.1612)	0.2321 ± 0.1474	0.2307(0.1272, 0.3342)^***	0.0183 ± 0.0209	−0.0116(−0.1068, 0.0837)
3	7	0.8024 ± 0.1298	0.0770(−0.0764, 0.2304)	0.0865 ± 0.1659	−0.1070(−0.2696, 0.0557)	0.00037 ± 0.00063	−0.0010(−0.1044, 0.1025)	0.0457 ± 0.0478	0.0159(−0.0794, 0.1111)
30	8	0.4182 ± 0.2373	−0.3072(−0.4554, −0.1590)^**	0.2812 ± 0.2147	0.0878(−0.0693, 0.2449)	0.1495 ± 0.1395	0.1481(0.0482, 0.2481)^**	0.0654 ± 0.1494	0.0356(−0.0564, 0.1276)
100	8	0.9111 ± 0.0301	0.1857(0.0375, 0.3339)^*	0.0124 ± 0.0279	−0.1810(−0.3381, −0.0239)^*	0.00034 ± 0.0003	−0.0010(−0.1010, 0.0990)	0.0257 ± 0.0141	−0.0042(−0.0962, 0.0879)
300	7	0.2290 ± 0.0850	−0.4964(−0.6498, −0.3429)^***	0.3402 ± 0.1644	0.1468(−0.0158, 0.3094)	0.3149 ± 0.1451	0.3135(0.2101, 0.4170)^***	0.0734 ± 0.1922	0.0436(−0.0517, 0.1388)
P-trend^§	0.18			0.66		0.04		0.30
		*Bacteroidetes*		*Planctomycetes*		*Actinobacteria*		*Cyanobacteria*
TCS exposure levels (ng/mL)	n	mean±SD	β(95%CI)	mean±SD	β(95%CI)	mean±SD	β(95%CI)	mean±SD	β(95%CI)
0(DMSO)	8	0.0262 ± 0.0119	Reference	0.008 ± 0.0043	Reference	0.0033 ± 0.0013	Reference	0.0124±0.0160	Reference
blank control	7	0.0201 ± 0.0318	−0.0062(−0.0557, 0.0434)	0.0071 ± 0.0156	−0.0009(−0.0253, 0.0235)	0.0026 ± 0.0043	−0.0007(−0.0117, 0.0103)	0.0086±0.0204	−0.0038(−0.0134, 0.0059)
0.03	6	0.0086 ± 0.0068	−0.0176(−0.0693, 0.0341)	0.0054 ± 0.0128	−0.0026(−0.0281, 0.0228)	0.0009 ± 0.0007	−0.0023(−0.0138, 0.0091)	3×10⁻⁶ ±8.16×10⁻⁶	−0.0124(−0.0224, −0.0023)^*
0.3	7	0.02 ± 0.01470	−0.0062(−0.0558, 0.0434)	0.002 ± 0.0039	−0.0060(−0.0304, 0.0184)	0.0019 ± 0.0016	−0.0014(−0.0124, 0.0096)	0	−0.0124(−0.0220, −0.0028)^*
3	7	0.037 ± 0.0207	0.0107(−0.0388, 0.0603)	0.0182 ± 0.0359	0.0102(−0.0142, 0.0346)	0.0083 ± 0.0070	0.0050(−0.0060, 0.0160)	9×10⁻⁶ ±2.46×10⁻⁵	−0.0124(−0.0220, −0.0028)^*
30	8	0.0429 ± 0.0851	0.0167(−0.0312, 0.0646)	0.0235 ± 0.0490	0.0155(−0.0081, −0.0390)	0.0187 ± 0.0259	0.0154(0.0048, 0.0026)^**	0	−0.0124(−0.0217, −0.0031)^*
100	8	0.0373 ± 0.0131	0.0111(−0.0368, 0.0589)	0.0054 ± 0.0080	−0.0026(−0.0261, 0.0210)	0.0077 ± 0.0045	0.0044(−0.0062, 0.0150)	0	−0.0124(−0.0217, −0.0031)^*
300	7	0.0355 ± 0.0918	0.0093(−0.0403, 0.0589)	0.0015 ± 0.0023	−0.0065(−0.0309, 0.0179)	0.0031 ± 0.0075	−0.0001 (−0.0111, 0.0109)	0	−0.0124(−0.0220, −0.0028)^*
P-trend^§	0.31			0.94		0.20		0.001

^§

P-trend for DMSO control and TCS concentrations from 0.03–300ng/ml.

P<0.0;

^**

p<0.01

^***

p<0.0001.