Table 1.
Characteristics of the applied vectors and generated CAR T cells
| Sample | MOIa | Particles/cell | VCNb | Viabilityc |
|---|---|---|---|---|
| Untransduced | – | – | n.d. | 62.7% |
| CD8-LV | 0.045 | 12 × 103 | 0.91 ± 0.71d | 69.3% |
| VSV-LV | 33.3 | 5.8 × 103 | 3.94 ± 1.76 | 74.6% |
n.d., non-detectable.
a
As determined on MOLT cells.
b
VCN was measured in replicates from different samples generated using different batches of LVs on three donors, including the batch and donor used in the scRNA-seq experiment (untransduced n = 3, CD8-LV n = 7, VSV-LV n = 14) (mean ± standard deviation).
c
Determined by BD Rhapsody scanner upon staining with calcein AM and DRAQ7, before single-cell seeding.
d
VCN was quantified on DNA extracted from whole samples and extrapolated on CD8 cells based on their frequency determined by flow cytometry.