Fig. 1.

Myeloid lineage contributed to pathological endothelium formation in mice with laser-induced CNV. (a) GFP bone marrow chimeric mice were subjected to laser-induced CNV model and choroid flat mounts at Day seven after laser were stained with macrophage/microglia marker, IBA1 (red). (b) GFP bone marrow chimeric mice were subjected to laser-induced CNV and retinal cross sections at Day seven after laser were stained with EC marker vWF (red). White dotted line indicated the CNV lesion areas on retinal cross sections; (c) 8-week-old myeloid-specific LysM Cre driven mTmG reporter mice were subjected to laser-induced CNV model and choroid flat mounts at Day seven after laser were stained with EC marker Collagen IV (cyan). White dotted line indicated the CNV lesion areas on choroid flat mount. mG: membrane GFP; mT: membrane Tomato red. Scale bar: 100µm in (a), 50µm in (b), and 20µm in (c).