Fig. 2.
SOCS3 was induced in myeloid cells and controlled laser-induced CNV. (a) Socs3 mRNA expression was highly induced in choroid with CNV lesions after laser exposure compared with normal choroid (n=8-20, *, p<0·05, ****, p<0·0001, unpaired nonparametric Mann-Whitney Test). (b-c) Immunohistochemistry staining on choroid flat mount of myeloid-specific report mice (CreLysM::mTmG) (c) and cross section of wild type mice (b) showed that SOCS3 (magenta in c, red in b) was induced in myeloid lineage cells labeled with GFP in CNV lesion area (white dotted line) (c) and in subretinal space (yellow dotted line) (b) at three hours, six hours or Day three after laser exposure; mG: membrane GFP; mT: membrane Tomato red. (d-e) Myeloid-specific Socs3 deficiency promoted laser-induced CNV (n=41-50 lesions) while myeloid specific Socs3 overexpression suppressed laser-induced CNV labeled by isolectin (red) at Day seven after laser exposure (n=27-31) (****, p<0·0001, unpaired nonparametric Mann-Whitney Test). (f) GFP bone marrow chimeric mice were generated using Socs3 cKO and control mice as recipient mice and laser-induced CNV were performed. The choroid flat mounts were collected on Day seven and isolectin IB4 (red) was stained for CNV lesion. Scale bar: 20µm in (c), 100µm in (b), 200µm in (d, e, and f, top panel), and 50µm in (d, e, and f, bottom panel).