Table 1.
Phytochemical class | Observations | Results |
---|---|---|
Alkaloids | Turbidity/precipitation | - |
Carbohydrates | Formation of a purple product at the interface of the two layers | + |
Flavonoid | Formation of yellow color, which changed to colorless on acid addition | +++ |
Steroids | Green to pink color was absent | - |
Terpenoids | Appearance of reddish brown-deep red color | - |
Cardiac Glycosides | Lower reddish-brown layer and upper acetic acid layer, which turns bluish green | + |
Tannins | Appearance of white precipitation | ++ |
Saponins | Stable froth formation | + |
(-) = not present; (+) = present in low concentration; (++) = present in moderately high concentration; and (+++) = present in very high concentration.