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. 2021 Oct 21;73:103641. doi: 10.1016/j.ebiom.2021.103641

Figure 4.

Figure 4

Pravastatin regulates intercellular junctions and improves epithelial integrity. (a) Transepithelial electrical resistance (TEER), (b) FITC-dextran, and (c) dispase-based dissociation assay in control (Con), pravastatin (PS)-treated, irradiated (IR), and IR+PS-treated Caco-2 monolayers. (d) Immunofluorescence of E-Cad, ZO-1, OCLN, and DSG2 in Con, PS, IR, and IR+PS-treated Caco-2 monolayers. (e) Western blot analysis and (f) mRNA levels of ZO-1, OCLN, and DSG2. (g) Western blot analysis of phosphorylated p38 MAPK (p-p38 MAPK) and toral p38 MAPK (p38 MAPK) in Con, PS, IR, and IR+PS-treated Caco-2 monolayers. Bar = 10 μm. One-way ANOVA was used to compare the results. Results are presented as mean ± standard error of the mean values; n = 3-4 per group. **P < 0.01, ***P < 0.001 compared to control group; #P < 0.05, ##P < 0.01, ###P < 0.001 compared to IR group. All data represent at least two independent experiments.