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. 2021 Oct 21;73:103641. doi: 10.1016/j.ebiom.2021.103641

Figure 8.

Figure 8

Pravastatin attenuates radiation-induced intercellular junction damage by activating metallothionein 2. (a) Immunofluorescence of ZO-1, OCLN, and DSG2 in control (Con), pravastatin (PS), si-MT2, and si-MT2+PS with/without irradiation (IR) treatment in Caco-2 monolayers. Bar = 10 μm. (b) Western blot analysis and (c) mRNA levels of ZO-1, OCLN, and DSG2 in Con, PS, si-MT2, and si-MT2+PS with/without IR treatment in Caco-2 monolayers. (d) Western blot analysis of phosphorylated p38 MAPK (p-p38 MAPK) and total p38 MAPK (p38 MAPK) in Con, PS, si-MT2, and si-MT2+PS-treated irradiated Caco-2 monolayers. One-way ANOVA was used to compare the results. Results are presented as mean ± standard error of the mean values; n = 3-4 per group. n.s., not significant; **P < 0.01, ***P < 0.001 compared to control group; ###P < 0.001 compared to IR group. All data represent at least two independent experiments.