Table 3.
Results from the linearity, within-run precision (day 1, n = 4), and recovery studies across the canine urine cortisol reportable range.
| Level | Spiked cortisol concentration, nmol/L (μg/dL) | Urine | ||
|---|---|---|---|---|
| Mean measured concentration, nmol/L (μg/dL) | Within-run CV (%) | Recovery (%) | ||
| L10 | 1,380 (50) | 1,424 (51.6) | 2.18 | 103 |
| L9 | 1,035 (37.5) | 1,098 (39.8) | 6.01 | 106 |
| L8 | 552 (20) | 662 (24.0) | 1.69 | 120 |
| L7 | 345 (12.5) | 439 (15.9) | 2.95 | 127 |
| L6 | 172 (6.25) | 234 (8.49) | 2.56 | 136 |
| L5 | 69 (2.5) | 102 (3.68) | 2.38 | 147 |
| L4 | 38.6 (1.4) | 55 (1.99) | 6.29 | 142 |
| L3 | 13.8 (0.5) | 23.5 (0.853) | 7.25 | 171 |
| L2 | 6.9 (0.25) | 12.8 (0.465) | 7.55 | 186 |
| L1 (matrix) | 0 | 0.9 (0.0325*) | 29.5 | NA |
| L0 (blank) | 0 | 0.69 (0.025) | 84.9 | NA |
NA = not applicable.
*
The intrinsic cortisol concentration of the urine matrix (0.0325 μg/dL) was neglected for the recovery and then for the bias computations.