FIG 1.

PGD₂/DP1 signaling has contrasting roles in different cell types. (a to c) Disease progression in WT, Lyz2-DP1^−/−, CX3CR1-DP1^−/−, and DP1^−/− mice infected with 700 PFU of rJHMV intracranially. Data are pooled from three independent experiments (n = 12 to 14 mice per group). A log rank Mantel-Cox test was used for survival analysis between Lyz2-DP1^−/−, CX3CR1-DP1^−/−, and DP1^−/− mice (**, P < 0.01). Mann-Whitney U tests were used to analyze weight (days 8 to 20) (P < 0.05) (b) and clinical score (days 8 to 18) (P < 0.05) (c) data between Lyz2-DP1^−/−, CX3CR1-DP1^−/−, and DP1^−/− mice. (d) Virus titers in the brains of mice at 3 dpi and 5 dpi show reduced virus loads in Lyz2-DP1^−/− compared to WT mice. Data represent the means ± SEM of results pooled from two independent experiments with 5 to 10 mice per group. Data were analyzed using Mann-Whitney U tests. ****, P < 0.0001. (e) Representative confocal images showing virus distribution in the brain, as assessed by staining for N protein. (f) Summary data, analyzed using Mann-Whitney U tests. Five sections from 3 individual mice were included in the analysis. Data are representative of results from 3 mice per group (means ± SEM). Bar, 50 μm.