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. 2021 Sep 7;12(5):e01097-21. doi: 10.1128/mBio.01097-21

FIG 1.

FIG 1

LMP1 activates the p62 gene promoter. (A) Transcription factor-binding sites on the promoter sequence spanning −1166 to −1781 of the human p62 gene. (B) Diagram showing the p62 promoter construct pGL3/p62(−1781/−1166)-Luc2 and its mutants. (C) LMP1, NF-κB, NRF2, and Pu.1 transactivate wild-type pGL3/p62(−1781/−1166)-Luc2. (D) Response of pGL3/p62(−1781/−1166)-Luc2 mutants to LMP1, NF-κB, and p38α. 293 cells in 24-well plates were transfected with 150 ng IRF4, NRF2, or Pu.1 and 150 ng p65 plus p50 (75 ng each), or 10 ng LMP1 and 40 ng pGL3/p62(−1781/−1166)-Luc2 or its mutants, and 10 ng Renilla luciferase, for duplicates. A dual-luciferase assay was performed 24 h after transfection. Consistent results were obtained from at least three independent repeats, and representative results are shown. The ability of the vector control to activate the promoter construct was set to 1. The statistical analysis was performed on the results to compare results for the wild-type promoter to those for the mutants. *, P < 0.05; ***, P < 0.01; n.s., not significant.