TABLE 2.
Summary of bottleneck responses
| Strain | Bottleneck diam |
Total no. of expts different from WT (% of expts)c | Gene products altered | CFU level (% WT) at 18 hpi; 24 hpid | ||
|---|---|---|---|---|---|---|
| 18 hpia (>5 μm)b | 24 hpi (>5 μm) | 48 hpi (>4 μm) | ||||
| ΔluxCDABEG (Δlux) | 2/4 | 3/3 | 4/5 | 9/12 (75) | No light | 117; 57 |
| ainS − | 1/4 | 3/4 | 1/3 | 5/11 | No C8 | 82; 94 |
| ainS− + ainS | 0/4 | 0/1 | 0/3 | 0/8 | ||
| luxI − | 3/5 | 5/6 | 3/4 | 11/15 (73) | No 3O-C6 | 142; 65 |
| luxI− + luxI | 0/5 | 0/3 | 1/4 | 1/12 | ||
| ainS − luxI − | 4/4 | 1/1 | 1/4 | 6/9 (67) | No C8 or 3O-C6 | 78; 36 |
| ainS−luxI− + ainS | 4/4 | 1/1 | 2/4 | 7/9 (78) | No 3O-C6 | 88; 36 |
| ainS−luxI− + luxI | 2/4 | 0/1 | 0/4 | 2/9 | No C8 | 115; 48 |
| luxR − | 2/4 | 1/2 | 3/5 | 6/11 (55) | No regulator | 102; 36 |
| luxR− + luxI | 2/3 | 1/1 | 3/4 | 6/8 (75) | No regulator + 3O-C6 | 90; 42 |
| ΔluxIR lacZp-luxCDABEG | 2/2 | 4/4 | 1/3 | 7/9 (78) | No regulator or 3O-C6 + light | 52; 46 |
a
Time during symbiotic development, hours postinoculation (hpi).
b
Criteria to define bottleneck 1 (BN1) response, operationally defined for each time point based on WT. Data are displayed as the number of experiments with criterion/total number scored.
c
Each experiment includes 8 to 14 light organ measurements per treatment.
d
Level of V. fischeri population as the percentage of CFU/ relative to WT-colonized light organs (see Fig. S1 in the supplemental material for full data set).