FIG 1.

Phylogenetic tree of methanol dehydrogenases. The evolutionary history was inferred using the neighbor-joining method. The optimal tree with a sum of branch length of 8.78582079 is shown. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Jones-Taylor-Thornton (JTT) matrix-based method and are in the units of the number of amino acid substitutions per site. The analysis involved 51 amino acid sequences. All ambiguous positions were removed for each sequence pair. In total, there were 717 positions in the final data set. Alcohol dehydrogenases of the ADH type (1 and 2b) were used as an outgroup. The arrow points to the purified protein. Evolutionary analyses were conducted in MEGA 7 (79).