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(A) UV-visible (UV-Vis) spectra of Nd-XoxF1 purified from Methylacidiphilum thermophilum AP8. (B) Characteristic features of the redox cofactor PQQ showing a peak at 356 nm and a shoulder at 400 nm. Washing multiple times in 20 mM PIPES buffer (pH 7.2) resulted in an increase in A280/A356 ratio due to dissociation of the PQQ cofactor.
