FIG 6.

Prdm1 mediates suppression of IFN-γ-mediated antimicrobial defense in neonatal intestinal epithelium. (A) Expression of Prdm1 in primarily intestinal epithelial cells isolated from neonates, but not from adult mice, and in cultured IEC4.1 cells as measured using qRT-PCR and Western blotting. Representative gel images from at least three independent experiments are shown. (B) Effects of Prdm1 knockdown or forced Prdm1 expression on IFN-γ-mediated decrease of infection burden and IFN-γ-triggered gene expression in IEC4.1 cells in response to C. parvum infection. Statistical significance (ANOVA test): *, P < 0.01 versus control siRNA-treated/empty vector-transfected and C. parvum-infected cells; #, P < 0.01 versus control siRNA-treated/empty vector-transfected and C. parvum-infected and IFN-γ-treated cells. (C) Knockdown of Prdm1 in neonatal enteroids enhanced IFN-γ-mediated antimicrobial defense, with a further decrease of infection burden (Cp18s level) and increase of Nos2 expression in IFN-γ-treated infected neonatal enteroids. Data are shown as the means ± SD from at least three independent experiments. Statistical significance (ANOVA test): *, P < 0.01 versus C. parvum-infected enteroids; #, P < 0.01 versus IFN-γ-treated and C. parvum-infected enteroids.