TABLE 1.
PAP activities of the TbTim50 wild type and mutantsa
| Protein | Activity (nmol/min/mg) |
|---|---|
| GST-TbTim50 (wild type) | 234 ± 44 |
| GST-TbTim50 D242A, D244A | 52.5 ± 30.5 |
| GST-TbTim50 D345A, D347A | 82 ± 20 |
| GST | 46 ± 28 |
a
PAP activity was measured using 1,2-dioctanoyl-sn-glycero-3-phosphate (DiC8 PA) as the substrate. Recombinant proteins were purified as described in the additional materials and methods in the supplemental material (Text S1). Specific activity was calculated from three independent experiments.