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. 2021 Sep 7;12(5):e01871-21. doi: 10.1128/mBio.01871-21

FIG 5.

FIG 5

In vitro evolution induces S. stipitis genome plasticity. (A) Schematics of laboratory evolution strategy. (B) S. stipitis growth curve in SC G+X liquid media at permissive (30°C) and restrictive (37°C) temperature. (C) Karyotype organization of S. stipitis colonies after 8 weeks of laboratory evolution at 30 and 37°C. Gross chromosomal rearrangements following laboratory evolution at 30°C (blue) and 37°C (magenta) are indicated. The low resolution of CHEF detects changes in smaller chromosomes more precisely than changes in larger chromosomes. (D) Serial dilution assay showing growth at 30 and 37°C of the following strains: parental (P), 30°C-evolved strains without (NO Rearrangements) or with (Rearrangements) and 37°C-evolved strains without (NO Rearrangements) or with (Rearrangements). The CHEF analysis strain number is indicated. *, Colonies with a fitness advantage compared to the parental strain.