FIG 2.

MERS-CoV N protein interacts with MOV10 and related cellular partners. Huh-7 cells were mock infected or infected with MERS-CoV at an MOI of 0.1 (Inf.) for 20 h. (A) Cell lysates were immunoprecipitated with anti-MOV10 antibody. (B) Cell lysates were immunoprecipitated with anti-MOV10 antibody with or without RNase treatment. (C) Schematic diagram of the interaction between MOV10 and selected cellular partners (yellow) involved in different RNA metabolism pathways (light blue). (D) Cell lysates were immunoprecipitated with anti-TIAR, anti-AGO2, or anti-UPF1 antibodies. (E) Cell lysates were immunoprecipitated with anti-ATP5B antibody. In all cases, protein presence in the original extract (input) or in the immunoprecipitated samples (IP) was detected by Western blotting using specific antibodies.