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. 2021 Sep 14;12(5):e01316-21. doi: 10.1128/mBio.01316-21

FIG 3.

FIG 3

MOV10 activity on MERS-CoV infected cells. (A) Huh-7 cells were transfected with negative-control siRNA (siNeg; black) or MOV10-specific siRNAs (siMOV10; green), and the accumulation of MOV10 mRNA was quantified by RT-qPCR at the indicated time points. (B) Viral gRNA (blue) and sgmRNA-N (red) accumulation, at the indicated time points, was quantified by RT-qPCR in cells transfected with siMOV10 or with siNeg. (C) Cytoplasmic extracts were obtained from cells transfected with siNEG or siMOV10 and mock infected (0) or infected with MERS-CoV at an MOI of 1 for 8, 18, and 24 hpi. MOV10 and MERS-CoV N protein were detected by Western blotting. GAPDH was used as a loading control. Numbers under the blots indicate the estimated levels of N protein, normalized to GAPDH levels and relative to nonsilenced cells at 18 hpi. (D) Virus titers obtained from Huh-7 cells transfected with siNeg (gray) or siMOV10 (green) and infected with MERS-CoV. The values are means from three independent infections; error bars represent SD. ***, P < 0.001.