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. 2021 Sep 14;12(5):e01316-21. doi: 10.1128/mBio.01316-21

FIG 4.

FIG 4

MOV10 activity on MERS-CoV infected cells overexpressing MOV10. (A) Huh-7 cells were transfected with an empty vector (pcDNA) or with a plasmid expressing MOV10 (pcDNA-MOV10) and infected with MERS-CoV at an MOI of 1. Viral gRNA (blue) and sgmRNA-N (red) were quantified by RT-qPCR at 24 hpi. (B) Cytoplasmic extracts were obtained from cells transfected with an empty vector (pcDNA) or with a plasmid expressing MOV10 (pcDNA-MOV10) and infected with MERS-CoV at a MOI of 1 for 18 and 24 hpi. MOV10 and MERS-CoV N protein were detected by Western blotting. GAPDH was used as a loading control. Numbers under the blots indicate the estimated levels of N protein, normalized to GAPDH levels and relative to pcDNA transfected cells at 18 hpi. (C) Virus titers obtained from Huh-7 cells transfected with an empty vector (pcDNA; gray) or with a plasmid expressing MOV10 (pcDNA-MOV10; green) and infected with MERS-CoV. The values are means from three independent infections; error bars represent SD. *, P < 0.05; **, P < 0.01.