FIG 6.

Effect of MOV10 absence on N protein interaction with related cellular partners. Huh-7 and MOV10-KO cells were mock infected or infected with MERS-CoV at a MOI of 1. (A) At the indicated time points, viral gRNA (blue), mRNA N (red), and MOV10 mRNA (gray) accumulation was analyzed by RT-qPCR. (B) In addition, cytoplasmic extracts were analyzed by Western blotting for detection of MOV10 and N protein. GAPDH was used as a loading control. Numbers under the blots indicate the estimated levels of N protein, normalized to GAPDH levels and relative to Huh-7 cells at 18 hpi. (C) Virus titers obtained in Huh-7 (blue) and MOV10-KO (red) cells infected with MERS-CoV. The values are means from three independent infections; error bars represent SD. *, P < 0.05. (D) MOV10-KO cells were mock infected or infected with MERS-CoV (Inf.) at an MOI of 0.1. At 20 hpi, cell lysates were obtained and immunoprecipitated with anti-TIAR, anti-AGO2, or anti-UPF1 antibodies. Proteins in the original extract (input) or in the immunoprecipitated samples (IP) were detected by Western blotting using specific antibodies.