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In vitro EGFR protein and mRNA expression level treated with siRNA-loaded Tds (aX-Td@bsiRNA) after 48 h analysed by Western blot (A), the semiquantitative analysis by Image J of the protein level (B) and RT-qPCR (C). All data are expressed as the mean ± SD, n = 3. ∗∗∗P < 0.001, ∗∗P < 0.01 versus free siRNA group. #P < 0.05, ##P < 0.01, ###P < 0.001 versus 2X-Td@2siRNA group. The significance of the differences was evaluated by two-tailed Student's t-test.
