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. 2021 Mar 24;6(2):e00045-21. doi: 10.1128/mSphere.00045-21

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Copyright © 2021 Russo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — Mucoviscosity of cohorts of classical K. pneumoniae (cKp) strains, partially virulent hypervirulent K. pneumoniae (_pvhvKp) strains, and fully virulent hypervirulent K. pneumoniae (_fvhvKp) strains. Bacterial strains were grown in either lysogeny broth (LB) or iron-chelated M9 minimal medium plus added trace elements (c-M9-te). The culture OD₆₀₀ was normalized to 1.0 and then centrifuged at 1,000 × g for 5 min. Mucoviscosity was recorded as the post/prespin OD₆₀₀ ratio. (A) cKp strain cohort; (B) _pvhvKp strain cohort; (C) _fvhvKp strain cohort; (D) all cohorts grown in LB; (E) all cohorts grown in c-M9-te. The horizontal line marks 0.15. Three independent assays were performed on different days for each strain, and the data were reported as means ± SD. ****, P < 0.0001 by two-way ANOVA.