TABLE 1.
Phenotypes of GRA12-related genes during acute and chronic infectiona
| Strain | Acute infection |
Chronic infection |
|||
|---|---|---|---|---|---|
| Replication rate of tachyzoites | % survival (2 × 106) | % survival (2 × 105) | Cyst burden (2 × 102) | Reactivation rate (2 × 102) | |
| PruΔku80 | 14 ± 1.5 | 0.0 | 12.5 | 918 ± 107 | 85 ± 4.8 |
| GRA12A | ND | ND | ND | 933 ± 86 | 82 ± 5.4 |
| Δgra12A | 13 ± 0.3 | 0.0 | 37.5 | 375 ± 63 ↓ | 49 ± 6.8 ↓ |
| GRA12B | 13 ± 1.7 | ND | ND | 924 ± 82 | 82 ± 4.1 |
| Δgra12B | 19 ± 0.5 ↑ | 8.3 | 62.5 ↑ | 1,470 ± 151 ↑ | 58 ± 6.3 ↓ |
| GRA12D | ND | ND | ND | 825 ± 81 | 92 ± 7.2 |
| Δgra12D | 16 ± 1.5 | 0.0 | 100 ↑ | 688 ± 96 | 45 ± 4.6 ↓ |
a
The percent survival, cyst burden, and reactivation rate are shown with different inocula (e.g., 2 × 106). ND, not done. The arrows denote significant increase (upward pointing arrow) or decrease (downward pointing arrow).