TABLE 2.
Genotypes of strains used or developed in this study
| Strain | ToxoDB (TgME49 locus) | Parent | Reference |
|---|---|---|---|
| PruΔku80Δhxgprta | PruΔku80::HXGPRT | 38 | |
| PruΔku80ΔhxgprtΔgra12A::HXGPRT | TgME49_220890 | PruΔku80::HXGPRT | This study |
| PruΔku80ΔhxgprtΔgra12A::GRA12AHAx3-FLAG | TgME49_220890 | PruΔku80ΔhxgprtΔgra12A::HXGPRT | This study |
| PruΔku80ΔhxgprtΔgra12B::HXGPRT | TgME49_275860 | PruΔku80::HXGPRT | This study |
| PruΔku80ΔhxgprtΔgra12B::GRA12BHAx3-FLAG | TgME49_275860 | PruΔku80ΔhxgprtΔgra12B::HXGPRT | This study |
| PruΔku80ΔhxgprtΔgra12C::HXGPRT | TgME49_275850 | PruΔku80::HXGPRT | This study |
| PruΔku80ΔhxgprtΔgra12C::GRA12CHAx3-FLAG | TgME49_275850 | PruΔku80ΔhxgprtΔgra12C::HXGPRT | This study |
| PruΔku80ΔhxgprtΔgra12D::HXGPRT | TgME49_308970 | PruΔku80::HXGPRT | This study |
| PruΔku80ΔhxgprtΔgra12D::GRA12DHAx3-FLAG | TgME49_308970 | PruΔku80ΔhxgprtΔgra12D::HXGPRT | This study |
a
This parental strain expresses GFP in bradyzoites under the lactate dehydrogenase gene 2 (LDH2) promoter.